Complete RNAseq alignment guide – from fastq to count table
Even with the advent of single-cell sequencing, bulk RNAseq remains a powerful (and relatively inexpensive) tool for comparing the transcriptional profiles of tissues, cultures, and sorted cells. Here I will show you how to analyze the data starting with fastq. Some familiarity with BASH/Linux command line is required. System requirements:Unix-based operating systemStar is memory hungry …
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